This second edition of Protein Purification provides a guide to the major chromatographic techniques, including non-affinity absorption techniques, affinity procedures, non-absorption techniques and methods for monitoring protein purity. The new edition of the book has been organized to encourage incremental learning about the topic, starting with the properties of water, progressing through the characteristics of amino acids and proteins which relate to the purification process. There is an overview of protein strategy and equipment, followed by discussions and examples of each technique and their applications.
The basic theory and simple explanations given in Protein Purification make it an ideal handbook for final year undergraduates, and postgraduates, who are conducting research projects. It will also be a useful guide to more experienced researchers who need a good overview of the techniques and products used in protein purification.
* Guide to the major techniques used in protein purification
* Includes flowcharts to help the reader slect the best purification strategy
* Contains step-by-step protocols that guide the reader through each technique and its use
* Includes exercises and solutions
About the Author
Table of Contents
1. Protein Purification Strategy and Equipment 2. The Groundwork 3. Non-Affinity Absorption Techniques Used to Purify Proteins 4. Affinity Procedures Used to Purify Proteins 5. Non-Absorption Techniques for Purifying Proteins 6. Methods for Monitoring the Purity of Protein Solutions Appendix 1. The common units used in biology Appendix 2. The answers to the exercises Appendix 3. Single letter code for amino acids Appendix 4. The list of suppliers